TY  - THES 
TI  - Desarrollo y validación de un método analítico por UPLC para la determinación de ampicilina en una matriz biológica obtenida en ensayos de permeabilidad in vitro.
PB  - Universidad Icesi
PY  - 2013
UR  - http://hdl.handle.net/10906/76984
AB  - Due to in Colombia has not been reported models of permeability in vitro as a tool
for predicting the oral bioavailability of new or existing active substances, nor has
driven the development of analytical methodologies to quantify them in complex
biological matrices from these models.
This reflects a problematic; not having these methodologies hinders the knowledge
of pharmacokinetics and limits the applicability of pharmaceutical strategies to
improve bioavailability of substances that have absorption problems such as the
beta-lactam antibiotics.
In this project, a quick and simple analytical method was developed using
Efficiency Ultra Performance Liquid Chromatography (UPLC) to quantify the
fraction permeated ampicillin in Caco-2 intestinal epithelial cells , in order to predict
in vivo absorption. Additionally, the analytical methodology was validated following
the guidelines established by the United States Pharmacopeia in the Validation of
compendial methods and by the Food and Drug Administration in the Guide for
Validation of Bioanalytical Methods, finding that it meets parameters of
performance such as selectivity, precision, accuracy and linearity.
KW  - In vitro
KW  - Validación
KW  - Farmacocinética
KW  - Farmacéutica
KW  - Antibióticos
KW  - Métodos de investigación en bioquímica
KW  - Biochemistry research
KW  - Ampicillin
KW  - Analytical methodology
KW  - Producción intelectual registrada - Universidad Icesi
ER  -