TY  -  JOUR 
TI  - Epidermal Growth Factor Receptor Mutation In Patients With Non-Small Cell Lung Carcinoma In Fundacion Valle Del Lili, A University Hospital Of Reference In Latin America
PB  - American Lung Association
PB  - Elsevier
PY  - 2016
issn 0041-0101
AB  - Epidermal Growth Factor Receptor (EGFR) exon 19-deletion and exon 21 L858R mutation is found in 10% of Non-Small Cell Lung
Carcinoma (NSCLC) in Caucasians and in 20 to 40% in Asians. The use of Tyrosine Kinase Inhibitors (TKI) in these genetic alterations has
demonstrated a greater response and progression free survival in 9-15 months. Objective: To describe the experience in EGFR
identification in Fundación Valle del Lili (FVL).
Materials and Methods
Between June/2013 and Sep/2015, 95 samples were analyzed. A microdissection of tumor areas was performed as well as DNA extraction
with QIAamp FFPE Kit(Qiagen®) and amplification of exons 18, 19, 20 and 21 by PCR. It was visualized through microfluidic electrophoresis
with Agilent Bioanalyzer® System.
Results
98 samples were analyzed. Amplified DNA was obtained in 97% (95) of the cases. The mean age was 65,4±12 years, 63,2% (60) females and
36,8% (35) males. The mutation was observed in 25% (24), exon 19 in 10,5% and exon 21 in 14,7%. 83,1% of the tumors were
adenocarcinomas, 70,5% in stage IV and 29% of the cases with a mutation was receiving TKI. Mortality was 14,7% .
KW  - Ciencias socio biomédicas
KW  - Medical sciences
KW  - Pulmonar
KW  - Carcinoma
KW  - Fundación Valle del Lili
KW  - Crecimiento epidérmico
UR  - http://hdl.handle.net/10906/81356
ER  -