TY  - JOUR 
TI  - Genetically-Engineered Pig-to-Baboon Liver Xenotransplantation: Histopathology of Xenografts and Native Organs
PB  - Public Library of Science
PY  - 2012
issn 1932-6203
AB  - Orthotopic liver transplantation was carried out in baboons using wild-type (WT, n = 1) or genetically-engineered pigs (a1,3-
galactosyltransferase gene-knockout, GTKO), n = 1; GTKO pigs transgenic for human CD46, n = 7) and a clinically-acceptable
immunosuppressive regimen. Biopsies were obtained from the WT pig liver pre-Tx and at 30 min, 1, 2, 3, 4 and 5 h posttransplantation.
Biopsies of genetically-engineered livers were obtained pre-Tx, 2 h after reperfusion and at necropsy (4–7
days after transplantation). Tissues were examined by light, confocal, and electron microscopy. All major native organs were
also examined. The WT pig liver underwent hyperacute rejection. After genetically-engineered pig liver transplantation,
hyperacute rejection did not occur. Survival was limited to 4–7 days due to repeated spontaneous bleeding in the liver and
native organs (as a result of profound thrombocytopenia) which necessitated euthanasia. At 2 h, graft histology was largely
normal. At necropsy, genetically-engineered pig livers showed hemorrhagic necrosis, platelet aggregation, platelet-fibrin
thrombi, monocyte/macrophage margination mainly in liver sinusoids, and vascular endothelial cell hypertrophy, confirmed
by confocal and electron microscopy. Immunohistochemistry showed minimal deposition of IgM, and almost absence of
IgG, C3, C4d, C5b-9, and of a cellular infiltrate, suggesting that neither antibody- nor cell-mediated rejection played a major
role.
KW  - Ciencias  socio biomédicas
KW  - Medical sciences
KW  - Ingeniería genética
KW  - Trasplante hepatico
KW  - biopsias
UR  - http://hdl.handle.net/10906/80893
ER  -