Caracterización de la estructura de las comunidades de bacterias endófitas establecidas en tejidos vegetativos de dos especies del género Oryza spp. (O. sativa y O. glumaepatula) mediante la técnica de PCR - DGGE basada en la amplificación del GEN 16S rARN

Abstract

Endophytic bacteria are organisms, which colonize the inside of plant tissues without causing harm. These microorganisms are involved in different processes such as biological nitrogen fixation, phytohormone production and resistance to the activity of pathogenic organisms, thus making them a novel alternative for the improvement of crop varieties. The objective of this project was to carry out a comparative analysis of the structure of bacterial communities present in vegetative tissues (leaves and stems) of a genotype Oryza glumaepatula (# 243) and two accessions of Oryza sativa (IR64 and Nipponbare) using the DGGE technique based on the amplification of a hypervariable fragment of the 16S rRNA gene. Two methodologies were used for the assessment of bacterial communities: 1) The classical microbiological method to isolate culturable bacteria, and 2) Direct extraction of bacterial DNA. In both cases, the bacterial enrichment method was included in the process in order to increase the abundance of bacterial strains. A total of 23 different morphotypes were obtained by the microbiological method. Of these, 10 morphotypes were isolated from the tissues of IR64, 6 from Nipponbare and 7 from O. glumaepatula.