Desarrollo de herramientas computacionales para la búsqueda de secuencias reguladoras de la transcripción en procariotas

This paper deals with a PERL program for tracking the DNA sequences that join transcription factors regulating the genetic expression of prokaryotic cells. The gene sets where obtained from their expressions under the same environmental conditions. The model organism was the lactococcus lactis, an organism for which we possess its sequenced genome in gene bank format. The program found a larger number of possible regulatory sequences in the 5’ flanking region of the genes. The number of possible regulatory sequences was also determined for the amount of genes that made up the set. The program also located flanking gene sequences which could also be involved in its regulation but at a translational level. The comparison of the results with patterns obtained experimentally, was done by means of nucleotide position weight matrices, getting around 50% regulating sequences which coincide with those reported in the databases, indicating good program prediction capability, if you account for the fact that most prokaryotic cell regulating sequences have yet to be characterized by experimental methods.